5CDD

Crystal Structure of Israel acute Paralysis Virus Pentamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	112.177, 274.248, 288.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.000 - 2.700
Rwork	0.244
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	70.000
High resolution limit [Å]	2.700
Number of reflections	240313
<I/σ(I)>	2.67
Completeness [%]	98.7
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1M HEPES pH 7.5, 20% (w/v) PEG 10,000, 8% Ethylene glycol