5CD8

Crystal structure of the NTD of Drosophila Oskar protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9641
Spacegroup name	P 32
Unit cell lengths	66.951, 66.951, 112.831
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.434 - 3.001
R-factor	0.1918
Rwork	0.189
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cd7
RMSD bond length	0.005
RMSD bond angle	0.913
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.055	0.022	0.456
Total number of observations	65587
Number of reflections	11302
<I/σ(I)>	12.5		4.1
Completeness [%]	99.8	99.1	99.7
Redundancy	5.8	5.8	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	20% PEG 400, 200mM Mg(AC)2, 100mM NaAC, pH 5.6