5CD7
Crystal structure of the NTD L199M of Drosophila Oskar protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.962, 38.307, 102.130 |
| Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
| Resolution | 37.734 - 2.502 |
| R-factor | 0.2172 |
| Rwork | 0.214 |
| R-free | 0.26990 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.075 | 0.049 | 0.428 |
| Total number of observations | 124860 | ||
| Number of reflections | 34100 | ||
| <I/σ(I)> | 12.4 | 4.9 | |
| Completeness [%] | 99.8 | 99.4 | 100 |
| Redundancy | 7.3 | 7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 17-22% PEG 3350, 150mM NaCl, 100mM NaAC pH 5.6 |
