5CD6
Crystal structure of a TPR-domain containing protein (BDI_1685) from Parabacteroides distasonis ATCC 8503 at 2.26 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95369,0.97937,0.97922 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.480, 167.480, 312.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.715 - 2.260 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20810 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.715 | 29.715 | 2.340 |
| High resolution limit [Å] | 2.260 | 4.850 | 2.260 |
| Rmerge | 0.077 | 0.038 | 0.441 |
| Rmeas | 0.106 | 0.053 | 0.608 |
| Total number of observations | 460901 | ||
| Number of reflections | 125291 | 24001 | 24163 |
| <I/σ(I)> | 7.11 | 14.2 | 2 |
| Completeness [%] | 96.1 | 95.9 | 98.7 |
| Redundancy | 1.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2000M calcium acetate, 20.0000% polyethylene glycol 1000, 0.1M Imidazole pH 8.0 |
