5CD3
Structure of immature VRC01-class antibody DRVIA7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.829, 73.747, 101.158 |
| Unit cell angles | 89.84, 89.40, 89.95 |
Refinement procedure
| Resolution | 49.542 - 2.900 |
| R-factor | 0.2367 |
| Rwork | 0.234 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.240 | 0.890 |
| Number of reflections | 41586 | |
| <I/σ(I)> | 3.84 | 1.1 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 292 | 0.16 M ammonium sulfate, 20% (w/v) PEG 4000, 20% (v/v) glycerol and 0.08 M sodium acetate, pH 4.6 |
