5CD2
The crystal structure of endo-1,4-D-glucanase from Vibrio fischeri ES114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.563, 67.489, 101.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.500 - 1.550 |
R-factor | 0.1374 |
Rwork | 0.135 |
R-free | 0.17600 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.025 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.104 | 0.602 |
Number of reflections | 56160 | |
<I/σ(I)> | 20.362 | 1.67 |
Completeness [%] | 99.8 | 96.8 |
Redundancy | 6.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2M MgCl2, 0.1M Tris:HCl, 20%(w/v) PEG8000 |