5CC4
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-01-16 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.108, 60.399, 38.013 |
| Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
| Resolution | 32.120 - 1.900 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.750 |
| Data reduction software | APEX |
| Data scaling software | APEX (2013/1 for Windows) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.027 | 0.151 |
| Total number of observations | 156506 | |
| Number of reflections | 11137 | |
| <I/σ(I)> | 25.61 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.67 | 8.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
