5CBP

Crystal Structure of Conjoint Pyrococcus furiosus L-asparaginase at 37 degree C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	90.555, 90.555, 190.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.836 - 2.358
R-factor	0.2035
Rwork	0.201
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ra9
RMSD bond length	0.008
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.358	2.360
Rmerge	0.084	0.760
Number of reflections	19854
<I/σ(I)>	38.9	2.3
Completeness [%]	99.9	99.9
Redundancy	13.3	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	310	0.2 M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate, 30%(v/v) 2-Propanol