5CBK
Crystal structure of the strigolactone receptor ShHTL5 from Striga hermonthica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.282, 102.282, 101.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.526 - 2.462 |
| R-factor | 0.1712 |
| Rwork | 0.168 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w06 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.754 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.527 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.732 | |
| Number of reflections | 14453 | |
| <I/σ(I)> | 53.5 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.3 | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.7 M sodium citrate, 0.01 M magnesium chloride, 0.05 M HEPES pH 7.5 |
