5CBH
Structural and Functional Characterization of a Calcium-activated Cation channel from Tsukamurella Paurometabola
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97902 |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.475, 116.475, 128.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.250 - 3.370 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.948 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmerge | 0.740 | |
| Number of reflections | 11662 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 96.2 | 98.3 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 200, magnesium chloride, cacodylate |
