5CB3
Structural Insights into the Mechanism of Escherichia coli Ymdb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.902, 42.059, 70.346 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 29.040 - 1.800 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.21726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1spv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.380 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.256 |
| Number of reflections | 15827 | |
| <I/σ(I)> | 37 | 7.3 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M potassium sodium tartrate tetrahydrate, 20%(w/v) polyethylene glycol 3350 |
