5CB2

the structure of candida albicans Sey1p in complex with GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	I 21 21 21
Unit cell lengths	76.566, 120.926, 190.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.26156
Rwork	0.260
R-free	0.29806
RMSD bond length	0.013
RMSD bond angle	1.639
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	19897
<I/σ(I)>	27.2
Completeness [%]	99.5
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	120 Mm DL-Malic acid pH 7.0, 16% w/v PEG 3350, 20 mM Bicine pH 9.0, 400 mM Magnesium chloride hexahydrate, 6% D-(+)-Trehalose dihydrate