5CB1

Apo enzyme of human Polymerase lambda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	95
Detector technology	CCD
Collection date	2012-09-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	I 41 2 2
Unit cell lengths	205.285, 205.285, 111.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.974 - 3.300
R-factor	0.233
Rwork	0.230
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.002
RMSD bond angle	0.671
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.420
High resolution limit [Å]	3.300	7.081	3.300
Rmerge	0.069	0.038	0.511
Total number of observations	203161
Number of reflections	18085
<I/σ(I)>	17.2
Completeness [%]	99.2	92.8	99.8
Redundancy	11.2	9.4	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M HEPES, 3.0M Sodium Chloride