5CB0
Crystal structure and functional implications of the tandem-type universal stress protein UspE from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 121.134, 121.134, 241.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 3.207 |
| R-factor | 0.2489 |
| Rwork | 0.243 |
| R-free | 0.30060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3olq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.393 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.900 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 16684 |
| <I/σ(I)> | 3.3 |
| Completeness [%] | 90.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 288 | 10% 1.4-dioxane, 1.6 M ammonium sulfate, 0.1 M MES (pH 6.5) |
