5CAM
Crystal Structure of the Cytoplasmic Domain of the Pseudomonas putida Anti-sigma Factor PupR (SeMet)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.491, 134.691, 34.903 |
| Unit cell angles | 90.00, 113.78, 90.00 |
Refinement procedure
| Resolution | 67.346 - 2.171 |
| R-factor | 0.1818 |
| Rwork | 0.178 |
| R-free | 0.21650 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AutoSol) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.700 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 14546 | |
| <I/σ(I)> | 18.2 | 1.7 |
| Completeness [%] | 97.2 | 82 |
| Redundancy | 6.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100mM Bis-Tris pH 6.0, 3M NaCl |
