5CAJ
Crystal structure of E. coli YaaA, a member of the DUF328/UPF0246 family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.664, 77.060, 184.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.910 - 1.650 |
R-factor | 0.1523 |
Rwork | 0.151 |
R-free | 0.17580 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.224 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.779 | |
Number of reflections | 150892 | |
<I/σ(I)> | 15.6 | 1.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 15% PEG 8000, 0.1 M dibasic sodium phosphate, 0.075 M monobasic sodium phosphate, 0.1 M sodium citrate 4.6, 0.1 M NaCl, 3% w/v benzamidine HCl |