5CA9
Structures of the candida albicans sey1p GTPase in complex with GDPAlF4-
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.272, 120.527, 190.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.800 |
R-factor | 0.2541 |
Rwork | 0.253 |
R-free | 0.27901 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.763 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 21774 | |
<I/σ(I)> | 43.9 | |
Completeness [%] | 99.0 | |
Redundancy | 10.28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 160 mM Lithium sulfate monohydrate, 80 mM Tris pH 8.5, 20% w/v Polyethylene glycol 3,350, 40 mM Ammonium acetate, 20 mM Sodium citrate tribasic dihydrate pH 5.6, 6% w/v Polyethylene glycol 4,000, 20 mM spermidine |