5CA9

Structures of the candida albicans sey1p GTPase in complex with GDPAlF4-

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	76.272, 120.527, 190.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.800
R-factor	0.2541
Rwork	0.253
R-free	0.27901
RMSD bond length	0.015
RMSD bond angle	1.763
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	21774
<I/σ(I)>	43.9
Completeness [%]	99.0
Redundancy	10.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	160 mM Lithium sulfate monohydrate, 80 mM Tris pH 8.5, 20% w/v Polyethylene glycol 3,350, 40 mM Ammonium acetate, 20 mM Sodium citrate tribasic dihydrate pH 5.6, 6% w/v Polyethylene glycol 4,000, 20 mM spermidine