5C9Y
Crystal structure of yellow lupine LlPR-10.1A protein partially saturated with trans-zeatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-04 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.81738 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.506, 57.918, 62.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.480 - 1.500 |
| Rwork | 0.181 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1icx |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1-743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.090 | 0.829 |
| Number of reflections | 21584 | |
| <I/σ(I)> | 21.96 | 2.45 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 7.16 | 7.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 1.8 M (NH4)2SO4, 0.1 M MES, PH 6.5 |
