5C9N
Crystal structure of GEMC1 coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.035, 70.574, 83.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.780 - 2.200 |
R-factor | 0.22385 |
Rwork | 0.223 |
R-free | 0.24876 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uii |
RMSD bond length | 0.010 |
RMSD bond angle | 1.246 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.800 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.871 |
Number of reflections | 15376 | |
<I/σ(I)> | 11.8 | 1.5 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Hepes buffer pH 7.5, 7% Ethanol, 10% 2-Methyl-2,4-pentanediol (MPD) 0.01 M Ethylene-diamine-tetra-acetic acid disodium salt dihydrate. |