5C9M
The structure of oxidized rat cytochrome c (T28A) at 1.362 angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.517, 52.567, 61.771 |
| Unit cell angles | 109.86, 92.93, 92.20 |
Refinement procedure
| Resolution | 57.960 - 1.362 |
| R-factor | 0.131 |
| Rwork | 0.129 |
| R-free | 0.16982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c0z |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.803 |
| Data reduction software | XDS (Jan. 10, 2014) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHENIX (1.8.1) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.962 | 1.398 |
| High resolution limit [Å] | 1.362 | 1.362 |
| Rmerge | 0.052 | 0.580 |
| Number of reflections | 76544 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 92.4 | 66.24 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 20 mg/ml protein, PEG 4000, isopropanol, sodium acetate, potassium ferricyanate |
