5C94
Infectious bronchitis virus nsp9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 123.426, 123.426, 123.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.534 - 2.438 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.23610 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.534 | 3.070 |
| High resolution limit [Å] | 2.438 | 2.438 |
| Rmerge | 0.089 | 0.456 |
| Number of reflections | 6258 | |
| <I/σ(I)> | 0.164 | 2.4 |
| Completeness [%] | 98.8 | 91.2 |
| Redundancy | 12.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.18 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate pH 6.5, 28%(w/v) polyethylene glycol 8000 |
