5C90
Staphylococcus aureus ClpP mutant - Y63A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9735 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.359, 126.237, 145.647 |
Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.18312 |
Rwork | 0.182 |
R-free | 0.20164 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sta |
RMSD bond length | 0.011 |
RMSD bond angle | 1.466 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 340047 | |
<I/σ(I)> | 16.6 | 3.8 |
Completeness [%] | 99.6 | 100 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 30% MPD, 100mM sodium acetate (pH 5.5), 20mM calcium chloride |