5C8P
Crystal structure of MoCVNH3 variant (Mo0v) in complex with (N-GlcNAc)3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-02 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.747, 50.598, 43.609 |
Unit cell angles | 90.00, 109.86, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.020 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.073 | 0.234 |
Number of reflections | 7630 | |
<I/σ(I)> | 13 | 3.5 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 4.69 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | ~8-10 % (w/v) PEG 3350, 0.1 M sodium phosphate-citrate buffer, pH 4.2, 0.2 M NaCl |