5C8L
Crystal Structure of the Bdellovibrio bacteriovorus Nucleoside Diphosphate Sugar Hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.947, 103.279, 51.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.20900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5c7q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.497 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.064 | 0.043 | 0.466 |
| Total number of observations | 201670 | ||
| Number of reflections | 35689 | ||
| <I/σ(I)> | 19.1 | ||
| Completeness [%] | 92.4 | 99.6 | 54.4 |
| Redundancy | 5.7 | 6.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.75-2.0 M ammonium sulfate, 0.1 M HEPES pH 7.0, and 0-0.5% PEG 8000 |
