5C8J

A YidC-like protein in the archaeal plasma membrane

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	180.250, 251.420, 96.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.774 - 3.502
R-factor	0.2555
Rwork	0.253
R-free	0.30240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	76.400
High resolution limit [Å]	3.500
Number of reflections	55459
<I/σ(I)>	8.9
Completeness [%]	99.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.08 M calcium acetate and 18% PEG3350