5C8J
A YidC-like protein in the archaeal plasma membrane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 180.250, 251.420, 96.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.774 - 3.502 |
R-factor | 0.2555 |
Rwork | 0.253 |
R-free | 0.30240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.498 |
Data reduction software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 76.400 |
High resolution limit [Å] | 3.500 |
Number of reflections | 55459 |
<I/σ(I)> | 8.9 |
Completeness [%] | 99.0 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08 M calcium acetate and 18% PEG3350 |