5C8H

Crystal structure of ORC2 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792604
Spacegroup name	P 61
Unit cell lengths	55.038, 55.038, 75.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.660 - 2.010
R-factor	0.196
Rwork	0.190
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	polyalanine coordinates derived from PDB entry 4XGC.
RMSD bond length	0.015
RMSD bond angle	1.499
Data reduction software	XDS
Data scaling software	Aimless (0.5.7)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.660	47.660	2.060
High resolution limit [Å]	2.010	9.000	2.010
Rmerge	0.077	0.030	1.030
Rpim	0.024	0.009	0.327
Total number of observations	96076	1011	6717
Number of reflections	8700
<I/σ(I)>	21.5	63.8	2.2
Completeness [%]	99.8	98.8	98.1
Redundancy	11	9.4	10.6
CC(1/2)	0.999	0.999	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG-3350, 0.2 M magnesium formate