5C8F
Crystal structure of light-exposed full-length Thermus thermophilus CarH bound to cobalamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 126.910, 126.910, 149.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.740 - 2.650 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jml |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 18067 | |
| <I/σ(I)> | 26.8 | 1.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.8 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 3.4 M NaCl, 0.1 M Bis-Tris pH 6 |
