5C8C
Crystal structure of recombinant coxsackievirus A16 capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-16 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 347.900, 347.900, 347.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.700 - 2.500 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.17200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.217 | 0.796 |
| Number of reflections | 244135 | |
| <I/σ(I)> | 11.3 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 1.8M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate (pH 5.0) |
