5C8B

Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 43 21 2
Unit cell lengths	82.500, 82.500, 156.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 2.700
R-factor	0.2117
Rwork	0.208
R-free	0.28700
RMSD bond length	0.016
RMSD bond angle	1.637
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.800
High resolution limit [Å]	2.700
Rmerge	0.093
Number of reflections	15705
<I/σ(I)>	16.67
Completeness [%]	99.2
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	PEG 1000, 150mM NaCl, MES, 2% Sacharose