5C86
Novel fungal alcohol oxidase with catalytic diversity among the AA5 family, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 123.710, 123.710, 52.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.530 - 1.510 |
| R-factor | 0.15207 |
| Rwork | 0.150 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eie |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.079 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.640 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.079 | 0.794 |
| Number of reflections | 72213 | |
| <I/σ(I)> | 18.1 | 3.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M imidazole, pH 8.0, 12% v/v polyethylene glycol 8.000 and 4.5% 1-butanol. |
