5C82
Crystal structure of Nourseothricin acetyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.978690 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.536, 44.641, 42.244 |
Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
Resolution | 15.280 - 2.200 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.22390 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.820 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.091 | 0.341 |
Number of reflections | 9075 | |
<I/σ(I)> | 21.4 | |
Completeness [%] | 98.7 | 90.8 |
Redundancy | 7.2 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297.15 | 0.1 M Carboxylic acids (0.2M Sodium formate; 0.2M Ammonium acetate; 0.2M Sodium citrate tribasic dihydrate; 0.2M Sodium potassium tartrate tetrahydrate; 0.2M Sodium oxamate), 0.1 M Buffer System 3(Tris base; BICINE) 8.5, 50 % v/v Precipitant Mix 4(25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350) |