5C7Z
AP2 Mu2 adaptin C-terminal domain complexed with integrin alpha-4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54182 |
Spacegroup name | P 64 |
Unit cell lengths | 125.650, 125.650, 74.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.000 - 2.770 |
R-factor | 0.1768 |
Rwork | 0.174 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bxx |
RMSD bond length | 0.019 |
RMSD bond angle | 2.084 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.062 | 0.325 |
Number of reflections | 17034 | |
<I/σ(I)> | 14.2 | 3.7 |
Completeness [%] | 97.2 | 80.8 |
Redundancy | 4.9 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 2.2M NaCl, 0.4M Na/K phosphate, 10MM DTT 0.1M MES pH 7.1, 15% glycerol, molar ratio of peptide to protein 3:1 |