5C7W
5'-monophosphate Z:P Guanine Riboswitch bound to hypoxanthine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.180, 52.180, 274.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.923 - 3.220 |
| R-factor | 0.2308 |
| Rwork | 0.228 |
| R-free | 0.27970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fe5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.802 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.590 | 3.340 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.083 | 0.537 |
| Number of reflections | 4107 | |
| <I/σ(I)> | 24.9 | 4.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.54 | 8.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 17% PEG 1000, 240 mM NH4OAc, 10 mM K-HEPES, 10 mM Co(NH3)6Cl3 |
