5C7G
Crystal Structure of the b1 Domain of Human Neuropilin-1 in complex with a bicine molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97967 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.280, 62.280, 85.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.278 - 1.450 |
R-factor | 0.1435 |
Rwork | 0.141 |
R-free | 0.17250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kex |
RMSD bond length | 0.008 |
RMSD bond angle | 1.267 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 1.490 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.049 | 0.527 |
Number of reflections | 30515 | |
<I/σ(I)> | 24.9 | 3.9 |
Completeness [%] | 99.5 | 100 |
Redundancy | 12 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG550 mme 5%, PEG 20 000, 60mM MgCl2, Na-Bicine 100 mM, pH 8.5 |