5C78
ATP-driven lipid-linked oligosaccharide flippase PglK in apo-inward state (1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 96.600, 114.420, 145.590 |
Unit cell angles | 68.44, 73.27, 68.67 |
Refinement procedure
Resolution | 27.928 - 2.900 |
R-factor | 0.2333 |
Rwork | 0.232 |
R-free | 0.26710 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.586 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.970 | |
High resolution limit [Å] | 2.900 | 12.590 | 2.890 |
Rmerge | 0.106 | 0.053 | 1.188 |
Rmeas | 0.117 | 0.059 | 1.309 |
Total number of observations | 1176105 | ||
Number of reflections | 206754 | 2743 | 4310 |
<I/σ(I)> | 8.9 | 26.72 | 1.31 |
Completeness [%] | 87.2 | 94.9 | 23 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.2 | 298 | Tris-HCl; MgAcetate; PEG400 |