5C78
ATP-driven lipid-linked oligosaccharide flippase PglK in apo-inward state (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.600, 114.420, 145.590 |
| Unit cell angles | 68.44, 73.27, 68.67 |
Refinement procedure
| Resolution | 27.928 - 2.900 |
| R-factor | 0.2333 |
| Rwork | 0.232 |
| R-free | 0.26710 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.586 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.970 | |
| High resolution limit [Å] | 2.900 | 12.590 | 2.890 |
| Rmerge | 0.106 | 0.053 | 1.188 |
| Rmeas | 0.117 | 0.059 | 1.309 |
| Total number of observations | 1176105 | ||
| Number of reflections | 206754 | 2743 | 4310 |
| <I/σ(I)> | 8.9 | 26.72 | 1.31 |
| Completeness [%] | 87.2 | 94.9 | 23 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 298 | Tris-HCl; MgAcetate; PEG400 |
