5C77
A novel protein arginine methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.375, 95.620, 58.407 |
| Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
| Resolution | 30.249 - 2.500 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c74 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.997 |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 15852 |
| <I/σ(I)> | 14.9 |
| Completeness [%] | 99.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2 M sodium acetate, 0.1 M sodium cacodylate (pH 6.5), 30% (w/v) PEG 8000 |
