5C74

Structure of a novel protein arginine methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	107.570, 107.570, 87.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.158 - 1.900
R-factor	0.1949
Rwork	0.193
R-free	0.23210
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.998
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900	1.900
Number of reflections	41006
<I/σ(I)>	38.3
Completeness [%]	99.8
Redundancy	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.2 M (NH4)2SO4, 0.1 M Bis-Tris (pH 5.5), 25% (w/v) PEG 3350