5C70

The structure of Aspergillus oryzae beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.29996
Spacegroup name	P 61 2 2
Unit cell lengths	110.320, 110.320, 480.722
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.100
R-factor	0.2003
Rwork	0.198
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.414
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.191	0.118	0.471
Total number of observations	421087
Number of reflections	32783
<I/σ(I)>	17.59
Completeness [%]	100.0	100	99.9
Redundancy	12.8	11.9	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	1 M Succinic acid