5C6R
Crystal structure of PH domain of ASAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 48.120, 48.120, 111.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.120 - 1.800 |
| R-factor | 0.21068 |
| Rwork | 0.208 |
| R-free | 0.25986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2da0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.999 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 22383 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 95.4 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 100 mM Na2HPO4, 100 mM Tris, pH 8.0 and 48% PEG400 (v/v) |
