5C6Q
Crystal structure of the apo TOPLESS related protein 2 (TPR2) N-terminal domain (1-209) from rice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.27821 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 58.971, 58.971, 171.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.710 - 3.251 |
| R-factor | 0.2151 |
| Rwork | 0.213 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zhe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 10544 | |
| <I/σ(I)> | 27.5 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.3 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG 3350, 0.2 M magnesium chloride, 0.1 M BIS-TRIS, pH 6.5 |
