5C6K
Bacteriophage P2 integrase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.004, 54.420, 38.610 |
| Unit cell angles | 90.00, 97.33, 90.00 |
Refinement procedure
| Resolution | 35.541 - 1.900 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aih |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.290 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | |
| Number of reflections | 34250 | |
| <I/σ(I)> | 11.6 | 2.4 |
| Completeness [%] | 97.9 | 96.2 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Sodium nitrate, 0.1 M BisTris propane pH7.5, 20 % (w/v) PEG 3350 |
