5C6A
Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-08-04 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.106, 60.078, 37.978 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 37.870 - 1.850 |
R-factor | 0.1666 |
Rwork | 0.165 |
R-free | 0.20330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.747 |
Data reduction software | APEX |
Data scaling software | SAINT |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.028 | 0.186 |
Total number of observations | 192906 | |
Number of reflections | 11995 | |
<I/σ(I)> | 27.12 | 5.16 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.24 | 7.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |