5C6A
Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-08-04 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.106, 60.078, 37.978 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 37.870 - 1.850 |
| R-factor | 0.1666 |
| Rwork | 0.165 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.747 |
| Data reduction software | APEX |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.028 | 0.186 |
| Total number of observations | 192906 | |
| Number of reflections | 11995 | |
| <I/σ(I)> | 27.12 | 5.16 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.24 | 7.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
