5C5W
1.25 A resolution structure of an RNA 20-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 39.870, 39.870, 156.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.290 - 1.250 |
| R-factor | 0.1798 |
| Rwork | 0.179 |
| R-free | 0.18790 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.290 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.100 | 1.383 |
| Number of reflections | 13567 | |
| <I/σ(I)> | 21.5 | 1.6 |
| Completeness [%] | 100.0 | 89.9 |
| Redundancy | 23.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | See manuscript for details. 2M ammonium sulphate, 0.2 M NaCl, 0.1 M cacodylate buffer. |
