5C5T

The crystal structure of viral collagen prolyl hydroxylase vCPH from Paramecium Bursaria Chlorella virus-1 - 2OG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	33.790, 156.950, 41.240
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	28.427 - 1.598
R-factor	0.1787
Rwork	0.177
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jig
RMSD bond length	0.016
RMSD bond angle	1.483
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1977)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.430
High resolution limit [Å]	1.598
Number of reflections	55206
<I/σ(I)>	13.55
Completeness [%]	98.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M [Imidazole; MES monohydrate (acid)], 0.1 M amino acids [0.2 M L-Na-Glutamate; 0.2 M Alanine (racemic); 0.2 M Glycine; 0.2M Lysine HCL (racemic); 0.2 M Serine (racemic)], 30 % PEGMME 550 PEG 20K pH 6.5 [Morpheus HT96 condition H1, Molecular Dimensions] COmplex generated by soaking crystals in mother liquor supplemented with 250 mM ZnSO4 and 100 mM 2-OG for a period of 16 hours.