5C5L
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-08-27 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.709, 58.135, 34.796 |
| Unit cell angles | 90.00, 98.79, 90.00 |
Refinement procedure
| Resolution | 34.390 - 1.750 |
| R-factor | 0.1459 |
| Rwork | 0.144 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.908 |
| Data reduction software | APEX |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.130 | 1.770 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.036 | 0.190 |
| Total number of observations | 103986 | |
| Number of reflections | 12299 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.11 | 5.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
