5C5K
Structure of the Pfr form of a canonical phytochrome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.399, 192.747, 225.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.800 - 3.310 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2O9C and 4Q0J |
RMSD bond length | 0.002 |
RMSD bond angle | 0.602 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.800 | 48.800 | 3.480 |
High resolution limit [Å] | 3.310 | 14.340 | 3.310 |
Rmerge | 0.242 | 0.056 | 3.616 |
Rpim | 0.054 | 0.015 | 0.793 |
Total number of observations | 1137231 | 12575 | 94099 |
Number of reflections | 55199 | ||
<I/σ(I)> | 22.4 | 124.9 | 1.4 |
Completeness [%] | 99.8 | 96.9 | 99.6 |
Redundancy | 20.6 | 15.5 | 21.1 |
CC(1/2) | 0.998 | 0.997 | 0.469 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | PEG 3350, sodium chloride, ethylene glycol, MOPS, ammonium acetate, fructose, glucose, sodium citrate |