5C5J
Poymerase Nucleotide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91596 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.450, 57.380, 110.640 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 36.909 - 2.100 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.078 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.909 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.606 |
| Number of reflections | 63454 | |
| <I/σ(I)> | 11.2 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | MPD |
