5C5C
Human metabotropic glutamate receptor 7, extracellular ligand binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.175, 101.203, 55.318 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.862 |
| R-factor | 0.2075 |
| Rwork | 0.206 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mq4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 5.050 | 1.860 |
| Rmerge | 0.054 | 0.031 | 0.781 |
| Rmeas | 0.059 | 0.034 | 0.852 |
| Rpim | 0.023 | 0.014 | 0.335 |
| Total number of observations | 297932 | ||
| Number of reflections | 44656 | ||
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 97.6 | 97.9 | 91.4 |
| Redundancy | 6.7 | 6.6 | 6 |
| CC(1/2) | 0.998 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 0.2 M K2PO4 |
