5C59
Crystal structure of the periplasmic region of MacB from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.144, 78.565, 137.922 |
Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
Resolution | 19.998 - 3.000 |
R-factor | 0.2514 |
Rwork | 0.247 |
R-free | 0.30590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 53737 |
<I/σ(I)> | 31.9 |
Completeness [%] | 96.3 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 75 mM Potassium phosphate monobasic, and 20% PEG8K |