5C56
Crystal structure of USP7/HAUSP in complex with ICP0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97852 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.879, 80.378, 151.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.460 - 2.685 |
R-factor | 0.219 |
Rwork | 0.215 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ylm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.478 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.685 | 5.810 | 2.685 |
Rmerge | 0.096 | 0.045 | 0.866 |
Rmeas | 0.105 | 0.050 | 0.945 |
Rpim | 0.042 | 0.021 | 0.374 |
Total number of observations | 166411 | ||
Number of reflections | 27389 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.8 | 98.4 | 100 |
Redundancy | 6.1 | 5.6 | 6.2 |
CC(1/2) | 0.996 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG3350, 0.2M Sodium Bromide |